Us8709455b2 sericin cationic nanoparticles for application. Silk is a fibre produced by the silkworm in production of its cocoon. Materials and methods bombyx mori silk cocoons were obtained from silkworms reared in controlled conditions at the. Then, deionized water was added onto them and these were autoclaved at 120c for 30 min. The effects of bombyx mori silk strain and extraction time. In the present study memory impairment was induced to rats by ip injection of.
For applicable result in clinical use, we also study the efficacy of sericin added to a standard antimicrobial cream, silver zinc sulfadiazine, for open wound care in the treatment of seconddegree burn wounds. Hydrolysis with trypsin at different concentrations, temperatures and treatment times is employed for extraction of sericin. We focused on extraction of sericin in the absence of chemical additives to obtain a safe, effective, inexpensive sericin powder. The extraction of sericin protein from silk reeling. In one embodiment, unique ph variationresistant alkaline ionic water is produced from a novel stronglyalkaline ionic water generator device, wherein the unique ph variationresistant alkaline ionic water is subsequently utilized as a. Effect of calcium chloride concentration on silk solubility was studied. Extraction and characterization of silk sericin 365 been extracted from four different sources of mulberry silk and the effects of various parameters on extraction of sericin using infrared dyeing machine and hthp machine are studied. Dec 23, 2010 the present study investigated the chemical properties and antityrosinase activities of ss silk sericin extracted from different thai silk strains via various extraction methods.
However, practically all industrial removal methods involve extraction with soaps and detergents. Cytotoxicity of sericin extracted from 4 different methods including heat, acid, alkali and urea treatments was further studied in this work in the system of pure sericin films. Pdf extraction and characterization of silk sericin. Boiling water extraction was considered based on the response surface methodology rsm in order to identify the important factors for the sericin extraction. It could be seen that acid and urea extracted sericin films showed high percentage of cell attachment at 92% and 88%, respectively. Keywords bombyx mori silk silk proteins sericin methods of extraction molecular mass distribution electrophoresis ftir analysis. Materials and methods sample collection cocoons of various inbred sericinjams were collected from national academy of agricultural science, suwon, korea. Since the source as well as the method of extraction can affect the properties of. The gummy sericin material has to be removed from raw silk in degumming procedure to give luster to silk. Extraction of silk sericin can be accomplished by the method with some modification according to khalifaa et al.
Dry the folded dish on a metal mesh tray in a mechanical convection oven for 6 hours 10 minutes at 100 102 c or for 1. Us8709455b2 us378,583 us2009378583a us8709455b2 us 8709455 b2 us8709455 b2 us 8709455b2 us 2009378583 a us2009378583 a us 2009378583a us 8709455 b2 us8709455 b2 us 8709455b2 authority us united states prior art keywords hair sericin nanoparticles locks cationic prior art date 20090615 legal status the legal status is an assumption and is not a legal conclusion. Materials fabrication from bombyx mori silk fibroin. Maceration, percolation and infusion techniques of. In addition, chemical coagulation and organic solvent precipitation methods are also generally employed. Different silk strains contain distinct ss with various amino acid compositions, which are significantly influenced by the extraction method used. The purified silk fibroin was characterized by ftir and thermoanalysis technique. In this study, we developed an effective technology for the extraction of sericin from silk cocoons by deep sea water dsw. It can be extracted by boiling cocoon in the elevated alkali and enzymatic solution or at conditions of high pressure and high temperature in water. In the present study different methods of extraction of sericin from silk fiber have been carried out high temperature and high pressure, sodium hydroxide and.
Exploring natural silk protein sericin for regenerative. Extraction and physicochemical biological characterization for biopharmaceutical applications. Sericin characteristics, such as purity, molecular weight, antioxidant activity and biochemical properties, vary accordaramwit ing to the method used et al. Preparation and characterization of sericin powder extracted. Methods of extraction of medicinal plants maceration in this process, the whole or coarsely powdered crude drug is placed in a stoppered container with the solvent and allowed to stand at room temperature for a. Sericin extraction from silk cocoons flow chart induction of alzheimers disease. Properties and antityrosinase activity of sericin from various. For each of the silk material formats, the raw silk from the cocoons first must be treated to remove the sericin.
Silk sericin ameliorates wound healing and its clinical efficacy in burn wounds. Extracted sericin is characterized and compared with standard sericin sample. Aramwit, p, kanokpanont, s, nakpheng, t, srichana, t 2010b the effect of sericin from various extraction methods on cell viability and collagen production. During extraction, solvents diffuse into the solid plant material and solubilise compounds with similar polarity. The extraction procedures are specific to the analytes of interest and vary by group of analytes and type of extraction media. Pdf properties and antityrosinase activity of sericin. These steps are included in the silk fibroin extraction protocol.
There are several methods to extract and purify silk fibroin protein. The effect of sericin from various extraction methods on cell. The potential use of sericin in food, drug and cosmetics applications will be also detailed, due to its valuable bioactive properties. The products resulting from commonly used procedures extraction in boiling water, alkaline extraction, and extraction in autoclave were. To do this, 50 gram silk cocoons were first cut into small pieces. A flow chart depicts the procedure of extraction, purification and fabrication of a pure sericin 3d hydrogel details see methods. Further development of silk sericin as a biomaterial. Extraction and characterization of silk fibroin from waste.
Silk sericin was extracted by using hot water degumming process. Extracted sericin from silk waste for film formation. One of the most widely used procedures for the removal of sericin degumming is sodium carbonate boiling andor autoclaving. Analytical methods nitrogen content of sericin powder. The most commonly used method for the extraction of sericin protein is the acidulation precipitation method.
Extraction and physicochemicalbiological characterization for biopharmaceutical applications. Sericin extraction with water at 120 c extraction was extraction yield of 21. D gal infection has been generally accepted to establish an aging model for brain aging or antiaging pharmacological research cui et al. The extraction of fibroin protein from bombyx mori silk. Previous studies have demonstrated that urea extraction of sericin yields the highest antityrosinase activity. Results showed that the purified silk fibroin mainly had irregular curly conformation. Sericin was extracted using a simple hightemperature process involving heating, condensation with molus alba, filtering with cotton cloth. The effect of sericin from various extraction methods. Typically, the fibroin is extracted from the silkworm cocoon by removal of sericin 23 and is then purified. Mar 20, 2017 essential oils are the liquids that are isolated from plants when introduced to solvents they are liquefied versions of the plants. The effects of bombyx mori silk strain and extraction time on.
This method is simple, but the recovery rate is not high 40%, and it needs higher requirements on equipment acidresistant. The longduration aqueous extraction at 50 c but not at 60 c appeared as a valid option for obtaining bmss products where the hydrothermally induced fragmentation of the polypeptidic components is minimized. The objective of this patent application is the applicationuse of sericin nanoparticles m cosmetic formulas for hair care, as an agent that bestowsgives gloss and softness and promotes maintenance of coloration in dyed hair. Extraction and application of natural silk protein sericin from bombyx. Dialysis method was used to purify the dissolved silk fibroin. Extraction methods used pharmaceutically involves the separation of medicinally active portions of plant tissues from the inactiveinert components by using selective solvents.
Feb 01, 2010 the present study investigated the chemical properties and antityrosinase activities of ss silk sericin extracted from different thai silk strains via various extraction methods. Silk sericin ameliorates wound healing and its clinical. The molecular weight of extracted sericin protein using pure water showed in general a size distribution varying from 20 up to 400 kda, with major peaks between. Sericin extracted by different methods can yield different amino acid compositions. A novel ultrasonic oscillationinduced sericin extraction method produces a highmolecularweight sericin solution from silk cocoon threads at room temperature. The extraction yields of sericin are shown in table i. Potential applications of silk sericin, a natural protein. Pdf extraction and characterization of silk sericin researchgate. A comprehensive guide to essential oil extraction methods. Going back to the appraisers reply to the freab panel, two distinct. We found that ss produced from various extraction methods. Properties and antityrosinase activity of sericin from various extraction methods article pdf available in biotechnology and applied biochemistry 552. The effect of sericin from various extraction methods on. Some of the latest extraction methods for aromatic plants include headspace trapping, solid phase micro extraction, protoplast extraction, micro distillation.
In the processing of extraction, sericin is prone to degradation, which consequently. Steam distillation, solvent extraction, co2 extraction, maceration, enfleurage, cold press extraction, and water distillation. Determination of fat food safety and inspection service. Silk sericin ss is a natural macromolecular protein derived from the silkworm bombyx mori. The diverse methods of extraction of sericin, its origin, and the variety of cocoon, due to the different strains of b. Safety assessment of silk proteins as used in cosmetics.
The collected cocoons were kept in room temperature and were used for protein extraction. Rapid gelation and injectability of the sericin hydrogel although genipin, which is less toxic than glutaraldehyde, is recently used as a crosslinker for generating scaffolds involving sericin 28, it was reported. However, the morphology of sericin powder obtained from different extraction methods and different drying processes showed different shapes by sem. Sericin is a protein created by bombyx mori silkworms in the production of silk. The aim of this study was to evaluate the effect of silk sericin, a protein from silkworm cocoon, on scratch wound healing in vitro. Cleaner process for extraction of sericin using infrared. The extraction conditions of sericin from two silk wastes, pieced cocoon and inferior knubbs were studied to find the optimum extraction conditions. Sericin can be extracted by various methods, for example, highpressure and hightemperature techniques or acid, alkaline, or enzyme techniques.
Urea extraction of ss was the only method that provided clearly. Food chemistry preparation and characterization of sericin. The mtt assay indicated that ss from all extraction. Pdf properties and antityrosinase activity of sericin from. Gas permeation through waterswollen sericin pva membranes. Cocoons from a fibroindeficient mutant silkworm, bombyx mori. The mtt assay indicated that ss from all extraction methods had no toxicity to mouse fibroblast cells at concentrations up to 40. Plant part used as starting material solvent used for extraction. The present study investigated the chemical properties and antityrosinase activities of ss silk sericin extracted from different thai silk strains via various extraction methods. The basic parameters influencing the quality of an extract are.
Table 1 presents a comparison of the conventional degumming methods. Extraction of sericin by hthp method is free of chemicals, but produces fumes, is messy, time consuming and may damage fibroin aramwit et al. Essential oils are the liquids that are isolated from plants when introduced to solvents they are liquefied versions of the plants. Review article extraction and antioxidant activity of.
Effect of thai silk sericin and its extraction methods on. The groups of analytes that have been evaluated thus far are listed below, along with the types of extraction media that have been evaluated and the determinative methods in which the corresponding performance data can. Sericin extraction yield was calculated by weight method, as shown in the following formula. May 2009 silk protein sericin preparation and its characterization 395 showed similar physical properties on uv and ir spectra due to the same functional groups on their amino acids. In this study, therefore, urea extraction of sericin was performed, as described by aramwit.
Pdf study compares the quality and quantity of sericin obtained from four. This problem can be overcome by using a multistage process. The extraction of fibroin protein from bombyx mori silk cocoon. Extraction of silk sericin using enzymes extraction is carried out by using enzyme alkylase 25 or with 22. Us20150033442a1 ultrasonic oscillationinduced sericin. Waste silk fibers were dissolved in calcium chloride aqueous solution. Sericin plus silk inspired skin care beauty products. Review article extraction and antioxidant activity of sericin. Sericin extract was coated onto cotton fabric by a paddrycure method. Preparation and characterization of sericin powder. We found that ss produced from various extraction methods has different molecular weights, zeta potential, particle size and amino acid content.
There are several methods for sericin extraction such as treatment with heat, acid, alkali, or urea. Again, land and site in this article are interchangeable, but technically land represents the undeveloped parcel, while site represents the parcel as if ready to be built on. Sericin promotes fibroin silk i stabilization across a phase. Sericin was extracted with ice cold ethanol to obtain crude extract. Archives of dermatological research, 305 7, 585594. An extraction method is utilized if there are no land sales to arrive at the site land value. Sericin promotes fibroin silk i stabilization across a. There are several methods for removing sericin in the socalled degumming process of cocoons.
Chemical engineering materials engineering sericin. Methods of extraction of medicinal plants maceration in this process, the whole or coarsely powdered crude drug is placed in a stoppered container with the solvent and allowed to stand at room temperature for a period of at least 3. Properties and antityrosinase activity of sericin from. Nov 20, 2014 a flow chart depicts the procedure of extraction, purification and fabrication of a pure sericin 3d hydrogel details see methods. The collected silkworm strain used in this study is shown in table 1. It consists mainly of two proteins, fibroin and sericin. The methods used to generate the material formats used in these studies are included within this protocol. Using these proteins natural propensity for macrophaseseparation, we have been able to control the concentration of sericin and determine the impact it has on gelation and. Silk sericin ss can accelerate cell proliferation and attachment.
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